1. Place phenol mix at 37^oC for at least 1 hour
2. Melt excised agarose fragment at 65^oC. for 10 minutes or until agarose is molten, then place molten agarose at 37^oC for 2 minutes
3. Add 0.5X volume of phenol mix and shake vigorously for 45 seconds
4. Centrifuge for 5 minutes at room temperature and transfer aqueous layer to clean tube
5. Repeat phenol extraction and centrifugation step
6. Transfer aqueous layer to a clean tube and add 1/50 volume of 5M NaCl, shake for 45 seconds, and centrifuge for 5 minutes at room temperature
7. Transfer aqueous material to a clean tube, add 2X volume of EtOH and precipitate (either 15 minutes at -70^oC. or at least 1 hour at -20^oC)
8. Centrifuge for 15 minutes and aspirate off aqueous material
9. Wash pellet in 1X volume of 70% EtOH and repeat centrifugation
10. Aspirate off aqueous material and air dry pellet for 5 minutes (or dry in speedvac for ~2 minutes)
11. Resuspend pellet in 20µl TE buffer

Phenol mix: 25ml pure phenol, 15ml H₂O, 400ml 1M Tris-HCl, pH to 8.0 with 2 drops 5M NaCl