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HOME > Protocols > Molecular Biology > Real-time PCR > Taqman - Human c-myc Protocol

Taqman - Human c-myc Protocol

Primers and Probe

c-myc Forward Primer
5'-TCAAGAGGTGCCACGTCTCC-3'

c-myc Reverse Primer
5'-TCTTGGCAGCAGGATAGTCCTT-3'

c-myc Probe
FAM-CAGCACAACTACGCAGCGCCTCC-TAMRA


Master Mix Recipe (use 5無 RT reaction)

c-myc Master Mix Components
Volume (無) per Reaction
Concentration in Final Reaction
H2O
22.75 無
---
10X TaqMan buffer A
5 無
1 X
25 mM MgCl2
11 無
5.5 mM
2% gelatin
1.25 無
0.05%
25 mM dNTPs
1 無
500 然
5 然 c-myc Probe
1 無
100 nM
10 然 c-myc Forward Primer
1 無
200 nM
10 然 c-myc Reverse Primer
1 無
200 nM
AmpErase UNG
0.5 無
0.01 U/無
AmpliTaq Gold
0.5 無
0.05 U/無


Thermal Cycler Conditions

Temperature
Time
Cycles
50蚓 2 minutes  
95蚓 10 minutes  
95蚓 15 seconds
40 cycles
60蚓 1 minute

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