Protocol for Fixation of Frozen Tissue Sections

1. Clean slides with 95% ethanol
2. Treat with a solution containing 0.3% gelatin and 0.05% chromium poatassium sulfate
3. Cut 6 to 8 micron thick cryostat sections of tissue block
4. Allow frozen sections to come to room temperature
5. Fix slides in cold acetone (-20^oC) for 10 minutes
6. Rinse three times with PBS