Protocol for Preparation of Paraffin Tissue Sections

1. Section paraffin blocks at 4-6 microns and float on a water bath containing only deionized or distilled water
2. Pick up sections on slide (poly-l-lysine or chrome alum coated)
3. Dry overnight at room temperature
4. Place in drying oven at 60^oC for 45 minutes prior to deparaffinization
5. Deparafinize in xylene for 10 minutes. Repeat twice for a total of three treatments
6. Hydrate sections through graded alcohols
7. Rinse three times with distilled water
8. Incubate for 10 minutes with 0.1% H₂O₂ in PBS to quench endogenous peroxidase activity
9. Rinse four times with PBS
10. Slides are ready for staining