Protocol for Thawing Serum

1. Remove serum bottles from the freezer and allow them to sit at room temperature for approximately 10 minutes (you don't want to stick them directly in the water bath as some bottles may be brittle)
2. Place bottles in a 30^oC water bath. Some people use 37^oC, but the higher the temperature the more degradation of nutrients. Make sure the bottles cannot tip over or otherwise become submerged in the water bath
3. Gently swirl the bottles every 10 minutes until thawed completely

Note: The key to not have a lot of precipitation here is to swirl the bottles regularly. If they are just allowed to thaw in the water bath then you are sure to precipitate some of the componenets of the serum.