AflII Restriction Enzyme

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AflII

Restriction Site Sequence:
...CTTAAG... ...GAATTC...

Restriction Site Sequence After Cut:
...C TTAAG... ...GAATT C...

Restriction enzyme reaction conditions:
37^oC

Compatible ends (at this website):
BfrI, BspTI, Bst98I, MspCI, Vha464I

Heat Denaturation:
AflII can be denatured by heating at 65°C for 20 minutes

Genomic source:
Anabaena flos-aquae is the genomic source for AflII