|  | 
  
 |  AlwI
 
 
  | Restriction Site Sequence: |  ...GGATCNNNNNN... ...CCTAGNNNNNN...
 |  
 
  
 | Restriction Site Sequence After Cut: |  ...GGATCNNNN 
 NN... ...CCTAGNNNNN N...
 |  
 
  
 | Restriction enzyme reaction conditions: |  37oC |  
 
 
  
 | Heat 
 Denaturation: |  AlwI can be denatured by heating at 65oC for 20 minutes |  
 |  |