|  AsuNHI
 
 
  | Restriction Site Sequence: |  ...GCTAGC... ...CGATCG...
 |  
 
  
 | Restriction Site Sequence After Cut: |  ...G 
 CTAGC... ...CGATC G...
 |  
 
  
 | Restriction enzyme reaction conditions: |  37oC |  
 
  
 | Compatible 
 ends (at this website): |  NheI |  
 
  
 | Heat 
 Denaturation: |  AsuNHI can be denatured by heating at 65°C for 20 minutes |  
 |