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 |  
 
   BbvI 
 
  
  
 
  | Restriction Site Sequence: | 
  
 ...GCAGCNNNNNNNNNNNNN... 
...CGTCGNNNNNNNNNNNNN... | 
 
  
   
  
 |  
  Restriction Site Sequence After Cut: 
  | 
  
 ...GCAGCNNNNNNNN 
 NNNNN... 
...CGTCGNNNNNNNNNNNN N... | 
 
  
   
  
 | Restriction enzyme reaction conditions: | 
  
 37oC | 
 
  
   
   
  
 | Heat 
 Denaturation: | 
  
 BbvI can be denatured by heating at 65oC for 20 minutes | 
 
  
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