BoxI Restriction Enzyme

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BoxI

Restriction Site Sequence:
...GACNNNNGTC... ...CTGNNNNCAG...

Restriction Site Sequence After Cut:
...GACNN NNGTC... ...CTGNN NNCAG...

Restriction enzyme reaction conditions:
37^oC

Compatible ends (at this website):
BstPAI, PshAI

Heat Denaturation:
BoxI can be denatured by heating at 80^oC for 20 minutes

Genomic source:
Microbacterium oxydans is the genomic source for BoxI