|  
 
   BsaAI 
 
  
  
 
  | Restriction Site Sequence: | 
  
 ...YACGTR... 
 ...RTGCAY... | 
 
  
   
  
 |  
  Restriction Site Sequence After Cut: 
  | 
  
 ...YAC 
 GTR... 
 ...RTG CAY... | 
 
  
   
  
 | Restriction enzyme reaction conditions: | 
  
 37oC | 
 
  
   
  
 | Compatible 
 ends (at this website): | 
  
 BstBAI | 
 
  
   
  
 | Heat 
 Denaturation: | 
  
 BsaAI can be denatured by heating at 80°C for 20 minutes | 
 
  
     |