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   BslI 
 
  
  
 
  | Restriction Site Sequence: | 
  
 ...CCNNNNNNNGG... 
 ...GGNNNNNNNCC... | 
 
  
   
  
 |  
  Restriction Site Sequence After Cut: 
  | 
  
 ...CCNNNNN 
 NNGG... 
 ...GGNN NNNNNCC... | 
 
  
  
 
  
 | Restriction enzyme reaction conditions: | 
  
  
 | 55oC | 
  
  
   
   
  
 | Heat 
 Denaturation: | 
  
 BslI can be denatured by heating at 80°C for 20 minutes | 
 
  
   
  
 | Genomic source:  | 
  
 Bacillus species is the genomic source for BslI | 
 
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