|  | 
  
 |  Bst6I
 
 
  | Restriction Site Sequence: |  ...CTCTTCNNNNN......GAGAAGNNNNN...
 |  
 
  
 | Restriction Site Sequence After Cut: |  ...CTCTTCN 
 NNNN... ...GAGAAGNNNN N...
 |  
 
  
 | Restriction enzyme reaction conditions: |  65oC |  
 
 
  
 | Heat 
 Denaturation: |  It is not konwn if Bst6I can be heat denatured |  
 |  |