PflFI Restriction Enzyme

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PflFI

Restriction Site Sequence:
...GACNNNGTC... ...CTGNNNCAG...

Restriction Site Sequence After Cut:
...GACN NNGTC... ...CTGNN NCAG...

Restriction enzyme reaction conditions:
37^oC

Compatible ends (at this website):
AspI, PsyI, Tth111I

Heat Denaturation:
PflFI can be denatured by heating at 65°C for 20 minutes

Genomic source:
Pseudomonas fluorescens F is the genomic source for PflFI