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HOME > Restriction Enzymes > SpeI Restriction Enzyme
SpeI

Restriction Site Sequence:
...ACTAGT...
...TGATCA...

Restriction Site Sequence After Cut:

...A CTAGT...
...TGATC A...

Restriction enzyme reaction conditions:
37oC

Compatible ends (at this website):
AhlI, BcuI

Heat Denaturation:
SpeI can be denatured by heating at 65°C for 20 minutes

Genomic source: 
Sphaerotilus natans is the genomic source for SpeI

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