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HOME > Restriction Enzymes > SphI Restriction Enzyme

Restriction Site Sequence:

Restriction Site Sequence After Cut:

...GCATG C...
...C GTACG...

Restriction enzyme reaction conditions:

Compatible ends (at this website):
BbuI, PaeI

Heat Denaturation:
SphI can be deantured by heathing to 65C for 20 minutes

Genomic source: 
Streptomyces phaeochromogenes is the genomic source for SphI

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