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SphI
| Restriction Site Sequence: |
...GCATGC...
...CGTACG... |
|
Restriction Site Sequence After Cut:
|
...GCATG
C...
...C GTACG... |
| Restriction enzyme reaction conditions: |
37oC |
| Compatible
ends (at this website): |
BbuI,
PaeI |
| Heat
Denaturation: |
SphI can be deantured by heathing to 65°C for 20 minutes |
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