|   
 
 
 
  | 
  
  
 |  
 
   TfiI 
 
  
  
 
  | Restriction Site Sequence: | 
  
 ...GAWTC... 
 ...CTWAG... | 
 
  
   
  
 |  
  Restriction Site Sequence After Cut: 
  | 
  
 ...G 
 AWTC... 
 ...CTWA G... | 
 
  
   
  
 | Restriction enzyme reaction conditions: | 
  
 65oC | 
 
  
   
  
 | Compatible 
 ends (at this website): | 
  
 PfeI | 
 
  
   
  
 | Heat 
 Denaturation: | 
  
 TfiI cannot be heat denatured | 
 
  
   
 
 
   
 
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