|  | 
  
 |  Van91I
 
 
  | Restriction Site Sequence: |  ...CCANNNNNTGG... ...GGTNNNNNACC...
 |  
 
  
 | Restriction Site Sequence After Cut: |  ...CCANNNN 
 NTGG... ...GGTN NNNNACC...
 |  
 
  
 | Restriction enzyme reaction conditions: |  37oC |  
 
 
  
 | Heat 
 Denaturation: |  Van91I can be denatured by heating at 65°C for 20 minutes |  
 
 
 |  |