|   
 
 
 
  | 
  
  
 |  
 
   ZrmI 
 
  
  
 
  | Restriction Site Sequence: | 
  
  
  ...AGTACT... 
 ...TCATGA... 
  | 
 
  
   
  
 |  
  Restriction Site Sequence After Cut: 
  | 
  
 ...AGT 
 ACT... 
 ...TCA TGA... | 
 
  
   
  
 | Restriction enzyme reaction conditions: | 
  
 37oC | 
 
  
   
  
 | Compatible 
 ends (at this website): | 
  
 AssI, ScaI | 
 
  
   
  
 | Heat 
 Denaturation: | 
  
 ZrmI can be denatured by heating at 65oC for 20 minutes | 
 
  
   
 
 
   
 
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