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HOME > Protocols > Media and Reagents > 2X YT Media Recipe (1000 ml)

2X YT Media Recipe (1000 ml)

  1. Measure ~900ml of distilled H2O.
  2. Add 16g Bacto Tryptone.
  3. Add 10g Bacto Yeast Extract.
  4. Add 5g NaCl.
  5. Adjust pH to 7.0 with 5N NaOH.
  6. Adjust to 1L with distilled H2O.
  7. Sterilize by autoclaving.


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