Reagent and Buffer Recipes

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3M K 5M Ac - Recipe for the preparation of 3M K 5M Ac.
4F1G - Recipe for 4% formaldehyde and 1% gluteraldehyde.

A
ACD - blood anticoagulant Recipe.
Acetic Lacmoid - Acetic lacmoid stain Recipe.
Acetic Orcein - Acetic orcein stain Recipe.
Aceto-Carmine - Solution used for chromosome staining.
Acid Alcohol Decolorizer - reagent used in acid fast staining.
Acridine Orange - a nucleic acid selective metachromatic stain useful for cell cycle determination.
Acyrlamide (30%) - Used for acrylamide gels (obviously)
Acrylamide (40%) - Used for acrylamide gels (obviously)
Albert stains - reagents used to demonstrate volutin granules and cytoplasm in diphtheria organisms.
Alkaline Lysis Buffer 2 - Recipe for the preparation of alkaline lysis buffer 2. Used for plasmid prep.
Alkaline Lysis Buffer 3 - Recipe for the preparation of alkaline lysis buffer 3. Used for plasmid prep.
Alkaline Lysis Buffer A - Recipe for the preparation of alkaline lysis buffer A (alkaline lysis buffer 1 after the addition of lysozyme). Used for plasmid prep.
Amido Black - Recipe for making amido black. Amido black is an amino acid dye used to detect blood. It stains the proteins in blood a blue-black color.

B
Bang's Reagent - Used for determination of glucose.
Barfoed's Solution - Used for the detection of monosaccharides in solution.
Benedict's solution - Used for glucose estimation
Best's Carmine - Bests Carmine stain Recipe used for selectively staining glycogen.
Blue green algae agar - Recipe for blue green algae agar.
Blue green algae broth - Recipe for blue green algae broth.
Bradford's Reagent - Protein assay dye.
Bromthymol Blue - Reagent used as an acidic indicator or as a measure of photosynthetic or respiratory activity.

C
Carmalum stain - used for cell nuclei.
CASO agar - Recipe for CASO agar.
Cell Staining Buffer - Cell staining buffer Recipe for use in staining cells prior to FACS analysis.
Coomassie Blue - Used for staining protein gels.
Corynebacterium agar - Recipe for Corynebacterium agar.

D
Denhardt solution (50x) - Recipe for the preparation of Denhardt solution 50x.
Denhardt solution (100x) - Recipe for the preparation of Denhardt solution 100x.
DEPC treated water - Recipe for the preparation of Diethylpyrocarbonate (DEPC) treated water.
Destain Solution - Recipe for the preparation of destaining solution.
DNA Loading Buffer (Orange G) - Better than Xylene cyanol for very low molecular weight DNA as it usually runs around the 20-30bp range on 1% agarose.
DNA Loading Buffer (XC/BB) - 10X Xilene Cyanol/Bromophenol Blue DNA loading buffer. Better for DNA of larger molecular weight.

E
ELISA blocking solution - Solution suitable for blocking for most ELISA assays.
ELISA coating buffer - Solution suitable for most ELISA coating steps.
EMB Media - Used for Detecting Sugar Fermentation
Ehrlich's Solution - Indol test reagent.

F
Field's Stain - a rapid stain used commonly on thin films for malarial parasites.
Fixation solution - Fixation solution for tissues.
Flagella Stain - allows for the direct observation of flagella.
Formaldehyde Gel Running Buffer - Recipe for the preparation of formaldehyde gel running buffer.
Fruit Fly media - General purpose media for fruit flies.

G
Gastric Juice Recipe - This solution is helpful when trying to determine the half-life of a drug product in gastric juice.
Gentian Violet Stain - Reagent used in the gram-stain procedure.
Giemsa stain - used on blood and bone marrow.
Gluconobacter agar - Recipe for Gluconobacter agar.
GTGB (20X) - Glycerol tolerant gel buffer (20X)

H
Hank's Buffered Salt Solution (HBSS) - Recipe for the preparation of Hank's Buffered Salt Solution (HBSS).
Hellings (10x) - Recipe for the preparation of 10X Hellings.
Homogenization Buffer - Recipe for Tissue Homogenization Buffer for ELISA.

K
Kovacs' Reagent - Indol test reagent.

L
LB Agar - Recipe for the preparation of LB agar bacterial media.
LB broth - Recipe for the preparation of LB broth bacterial media.
LB broth (low salt) - Recipe for the preparation of low salt LB broth bacterial media.

M
M17 media - Recipe for the preparation of M17 media.
M9 minimal media - Minimal salts bacterial media.
Malachite Green - Malachite green is a relatively weakly binding dye for staining bacterial spores. A decolorizer is not necessary for this dye.
Mallory's Triple Stain - A reagent especially suitable for studying connective tissue.
Mannitol agar - Recipe for mannitol agar.
Mannitol broth - Recipe for mannitol broth.
Marine agar - Recipe for marine agar. Used for the growth of several marine bacteria.
Marine broth - Recipe for marine broth. Used for the growth of several marine bacteria.
Masson's stain - a trichrome stain for connective tissue.
Melzer's Reagent - a stain used to study the cellular structure of fungi.
Methyl Green - a commonly used counterstain that stains cell nuclei light green.
Methyl Orange - a commonly used indicator dye that changes color in acidic conditions from red (at pH 3.1) to orange-yellow (at pH 4.4).
MRS media - Recipe for MRS media. MRS media has been used for the recovery of lactic acid bacteria (LAB) from various food products.

N
NADI reagent - commonly used as a test for indophenol oxidase.
Nutrient agar - Recipe for nutrient agar suitable for growth of many bacterial species.
Nutrient broth - Recipe for nutrient broth suitable for growth of many bacterial species.
NZCYM - NZ amine, NaCl, bacto-yeast extract, casamino acids, and magnesium sulfate.
NZM - NZ amine, NaCl, and magnesium sulfate.
NZYM - NZ amine, NaCl, bacto-yeast extract, and magnesium sulfate.

P
Pancreatic juice - useful if you need to know the half-life of a drug product in pancreatic juice.
Papanicolaou's stain - used commonly for cancer screeing and smear preparations of various bodily secretions.
Paraformaldehyde (2%) - Recipe for 2% Paraformaldehyde.
PBS (1X) - Phosphate buffered saline (1X)
PBS (10X) - Phosphate buffered saline (10X)
PBST (1X) - Phosphate buffer saline tween-20.
Phenol red lactose broth - turns yellow when lactose is fermented.

R
Red blood cell (RBC) lysis buffer - Recipe for the preparation of red blood cell (RBC) lysis buffer.

S
Schiff's reagent - structures rich in polysaccharides, mucopolysaccharides, or glycolipids are the principal tissues stained with Schiff's reagent after acid degreadation.
SOB media - Tryptone/yeast extract bacterial media.
SOC media - Tryptone/yeast extract bacterial media.
SSC (20X) - Sodium chloride and sodium citrate.
SSPE (20X) - NaCl, NaH2PO4-H2O, and EDTA
STET - Sucrose, triton x-100, EDTA, and sodium Azide.
Synthetic sea water - Recipe for synthetic sea water. Used as a base for growth media for an assortment of marine bacteria.

T
TAE (50X) - Tris, acetic acid, and EDTA.
TBE (10X) - Tris, boric acid, and EDTA.
TBS (1X) - Tris buffered saline.
TBST - Tris buffered saline with tween-20.
TE (1X) - Tris and EDTA.
Terrific broth - Recipe for the preparation of terrific broth bacterial media.
TES - Tris, EDTA, and sodium chloride.
TGB (5X) - Tris glycine buffer (5X).
TNE (1X) - Tris and NaEDTA.
TPE - Recipe for the preparation of Tris-phosphate-EDTA.
Trypan blue - Recipe for the preparation of trypan blue.
TSY agar - Trypticase soy yeast agar Recipe.
TSY broth - Trypticase soy yeast broth Recipe.
TTE (1X) - Recipe for the preparation of 1X TTE buffer.

W
Western transfer buffer - Recipe for the preparation of Western transfer buffer.

X
X-gal staining solution - Solution that can be used for staining x-gal containing tissues.

Y
YMG agar - Recipe for yeast and malt extract with glucose agar. This agar is used for a number of Streptomyces species.
YMG broth - Recipe for yeast and malt extract with glucose media. This media is used for a number of Streptomyces species.
YPD Agar - Yeast extract/peptone/dextrose bacterial agar.
YPD Media - Yeast extract/peptone/dextrose bacterial media.
YPG Media - Yeast extract/peptone/galactose bacterial media.
YT Media (2x) - Yeast extract/tryptone bacterial media.