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HOME > Protocols > Media and Reagents > 10X Phosphate Buffered Saline (PBS) Recipe

10 X Phosphate Buffered Saline (PBS) Recipe

  1. Dissolve the following in 800ml distilled H2O.
      • 80g of NaCl
      • 2.0g of KCl
      • 14.4g of Na2HPO4
      • 2.4g of KH2PO4
  2. Adjust pH to 7.4.
  3. Adjust volume to 1L with additional distilled H2O.
  4. Sterilize by autoclaving.

Related: 1X PBS Recipe, PBST Recipe


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