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HOME > Protocols > Media and Reagents > 20X SSPE Recipe

20X SSPE Recipe

  1. Dissolve the following in 800ml of distilled H2O.
      • 175.3g of NaCl
      • 27.6g of NaH2PO4-H2O
      • 7.4 g of EDTA
  2. Adjust the pH to 7.4 with 10N NaOH.
  3. Adjust the volume to 1L with additional distilled H2O.
  4. Sterilize by autoclaving.

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