ACD Blood Anticoagulant Recipe

I have been there before. You need to take some blood for your protocol and you have no anticoagulant tubes. Here is a quick protocol to make ACD anticoagulant.

1. Dissolve 1.32g of sodium citrate in 85ml of distilled water
2. Dissolve 0.48g of citric acid in the solution from step 1
3. Dissolve 1.47g of dextrose in the solution from step 2
4. Add distilled water to 100ml
5. Filter sterilize through 0.2um filter
6. Use 0.25ml of solution for 1ml of blood