Home | Jobs | News & Articles | Job Advice | Search | Protocols | Fun | @RealLabRat

Lab Rat with DNA

Post your resume
Candidates - post your resume

Search job listings
Candidates - search biotech jobs

Post job openings
Employers - post job openings

HOME > Protocols > Media and Reagents > Acetamide Medium Recipe

Acetamide Medium Recipe

  1. Dissolve the following in 1L of distilled water to make Trace Elements solution:
    • CoCl2 x 6 H2O 3.7 g
    • ZnSO4 x 7 H2O 1.4 g
    • MnSO4 x H2O 1.6 g
    • FeSO4 x 7 H2O 5.0 g
  2. Dissolve the following in 800 ml of distilled water to make Trace Elements solution:
    • Trace element solution* 1.0 ml
    • KH2PO4 15.0 g
    • Glucose 20.0 g
  3. q.s. to 1L with distilled water
  4. Autoclave to sterilize.
  5. After autoclaving add the following:
    • 1 M CSCl2 12.5 ml
    • 1 M Acetamide 10.0 ml
    • 1 M CaCl2 x 2 H2O 4.1 ml
    • 1 M MgSO4 x 7 H2O 2.4 ml


theLabRat.com 2005. All Rights Reserved.