Acetic Orcein Stain Recipe

1. Add 1g of Orcein to 45ml of glacial acetic acid
2. Heat solution from step 1 to dissolve
3. Slowly add solution from step 2 into 55ml of distilled water
4. Allow to cool and store

Cautions: ALWAYS use caution and wear personal protective equipment when in the laboratory with concentrated acids (or any other time)! Always add acid slowly into water and do not add water into acid!