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HOME > Protocols > Media and Reagents > Acetobacter Medium Recipe

Acetobacter Medium Recipe

    1. Combine the following in 700ml water:
      • 15g Agar
      • 1ml Vitamin solution (see below)
      • 1ml Trace element solution (see below)
      • 5g Yeast extract
      • 0.25g MgSO4 x 7 H2O
      • 1g KH2PO4
      • 3.3g (NH4)2SO4
    2. Autoclave to sterilize.
    3. Add 200ml of glucose solution (see below)
    4. Adjust to 1000ml with sterile distilled water

Vitamin solution:

  1. Combine the following in 100ml distilled water:
    • 0.2mg Biotin
    • 0.2mg Folic acid
    • 20mg p-Aminobenzoic acid
    • 20mg Riboflavin
    • 40mg Calsium pantothenate
    • 40mg Thiamine hydrochloride
    • 40mg Pyridoxine hydrochloride
    • 40mg Nicotinic acid
    • 200mg m-Inositol
  2. Autoclave or filter sterilize.

Trace element solution:

  1. Combine the following in 100ml distilled water:
    • 7.8mg CuSO4 x 5 H2O
    • 101mg MnSO4 x H2O
    • 178mg ZnSO4 x 7 H2O
    • 23.4mg Na2MoO4 x 2 H2O
    • 366mg FeSO4 x 7 H2O
    • 1.457g CaCl2 x 2 H2O
  2. Autoclave or filter sterilize.

Glucose solution:

  1. Combine the following in 1000ml distilled water:
    • 200g glucose
  2. Filter sterilize.

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