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HOME > Protocols > Media and Reagents > Alcoholic Bouin’s Fixative Recipe

Alcoholic Bouin’s Fixative Recipe

  1. Stock solution (stable for approximately 1 year)
    • Measure 750ml of 80% ethanol
    • Add 300ml of Formaldehyde
    • Slowly add 5g of Picric acid
  2. Working solution (prepare right before use)
    • 70ml of stock solution
    • 5ml of acetic acid
  • Caution: Picric acid can become explosive if allowed to dry out and is toxic through skin
    exposure. Formaldehyde is a carcinogen. Use personal protective equipement, prepare under hood in a well ventilated area. Consult with appropriate safety officials at your institution for proper procedures and disposal.

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