Alkaline Lysis Buffer A Recipe

1. Add the following to 900ml distilled H₂O
• 9g Glucose
• 3g Tris
• 20ml of 0.25M EDTA
2. q.s. to 1000ml with distilled H₂O
3. Autoclave using standard conditions
4. Allow to cool to room temperature
5. Store at 4^oC

When ready to use add 0.02g lysozyme to 100ml of alkaline lysis buffer A to yield alkaline lysis buffer 1.

Related links: Alkaline Lysis Buffer 2, Alkaline Lysis Buffer 3