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HOME > Protocols > Media and Reagents > Alkaline Lysis Buffer A Recipe

Alkaline Lysis Buffer A Recipe

  1. Add the following to 900ml distilled H2O
      • 9g Glucose
      • 3g Tris
      • 20ml of 0.25M EDTA
  2. q.s. to 1000ml with distilled H2O
  3. Autoclave using standard conditions
  4. Allow to cool to room temperature
  5. Store at 4oC

When ready to use add 0.02g lysozyme to 100ml of alkaline lysis buffer A to yield alkaline lysis buffer 1.

Related links: Alkaline Lysis Buffer 2, Alkaline Lysis Buffer 3

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