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HOME > Protocols > Media and Reagents > B5 Fixative Recipe

B5 Fixative Recipe

  1. Stock solution (stable for approximately 1 year). Mix well and do not use any metal utensils or foil lined lids.
    • 12.0g Mercuric chloride
    • 2.5g Sodium acetate
    • 200ml Distilled water 200 ml
  2. Working solution (prepare right before use)
    • 20ml of stock solution
    • 2ml of formaldehyde
  • Caution: Mercuric chloride is a severe skin and eye irritant.. Formaldehyde is a carcinogen. Use personal protective equipement, prepare under hood in a well ventilated area. Consult with appropriate safety officials at your institution for proper procedures and disposal.

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