B5 Fixative Recipe

1. Stock solution (stable for approximately 1 year). Mix well and do not use any metal utensils or foil lined lids.
   • 12.0g Mercuric chloride
   • 2.5g Sodium acetate
   • 200ml Distilled water 200 ml
2. Working solution (prepare right before use)
   • 20ml of stock solution
   • 2ml of formaldehyde

• Caution: Mercuric chloride is a severe skin and eye irritant.. Formaldehyde is a carcinogen. Use personal protective equipement, prepare under hood in a well ventilated area. Consult with appropriate safety officials at your institution for proper procedures and disposal.