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HOME > Protocols > Media and Reagents > Blue Green Algae Agar Recipe

Blue Green Algae Agar Recipe

    1. Dissolve the following in 850ml of distilled water:
        • 1.5g NaNO3
        • 0.04g K2HPO4
        • 0.075g MgSO4-7H2O
        • 0.036g CaCl2-2H2O
        • 0.006g Citric acid
        • 0.006g Ferric ammonium citrate
        • 0.001g EDTA
        • 0.02g Na2CO3
        • 1.0ml Trace metal mix
        • 10g Agar
    2. Adjust pH to 7.1
    3. Bring to 1000ml with distilled water
    4. Autoclave or filter sterilize.

Trace metal mix

    1. Dissolve the following in 850ml of distilled water:
      • 2.86g H3BO3
      • 1.81g MnCl2-4H2O
      • 0.222g ZnSO4-7H2O
      • 0.39g Na2MoO4-2H2O
      • 0.079g CuSO4-5H2O
      • 0.0494g Co(NO3)2-6H2O

Related: Blue Green Algae Broth Recipe

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