Bouin's Fixative Recipe

1. Stock solution
   • 3L Saturated picric acid
   • 1L Formaldehyde
   • 200ml Glacial acetic acid

• Note: Small biopsies fix in 2 to 4 hours, large specimens may need up to 3 days.
• Caution: Picric acid can become explosive if allowed to dry out and is toxic through skin
  exposure. Formaldehyde is a carcinogen. Glacial acetic acid is caustic. Use personal protective equipement, prepare under hood in a well ventilated area. Consult with appropriate safety officials at your institution for proper procedures and disposal.