Bradford's Reagent Recipe

Bradford's Reagent is used for determination of protein concentration. Bradford's reagent reacts primarily with arginine residues and secondarily with a few other amino acids. This makes it good for the quick estimation of protein concentration in a crude mixture, but not very good for estimation of a purified protein that is acidic or basic.

1. Dissolve 50mg of Coomassie Blue G250 in 50ml of methanol
2. Add 100ml of 85% H₃PO₄ to the solution from step 1
3. Add the solution from step 2 into 500ml of H₂O and mix
4. Filter to remove and precipitates
5. Add an additional 350ml of H₂O
6. Store at 4^oC.

Cautions: Always add acid slowly into water and do not add water into acid!