Bradford's Reagent is used for determination of protein
concentration. Bradford's reagent reacts primarily with arginine
residues and secondarily with a few other amino acids.
This makes it good for the quick estimation of protein
concentration in a crude mixture, but not very good
for estimation of a purified protein that is acidic
Dissolve 50mg of Coomassie Blue
G250 in 50ml of methanol
- Add 100ml of 85% H3PO4 to
the solution from step 1
- Add the solution from step 2 into 500ml of H2O
- Filter to remove and precipitates
- Add an additional 350ml of H2O
- Store at 4oC.
Cautions: Always add acid slowly into water and do
not add water into acid!