- Add 10g of carmalum to 200ml of distilled water
- Slowly add in 5ml of glacial acetic acid
- Boil for approximately 1 hour
- Allow to cool
Carmalum stain is used for cell nuclei.
Cautions: ALWAYS wear personal protective equipment
when in the laboratory with concentrated acids (or any
other time)! Always add acid slowly into water and do
not add water into acid!