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HOME > Protocols > Media and Reagents > Carmalum Stain Recipe

Carmalum Stain Recipe

Stock solution

  1. Add 10g of carmalum to 200ml of distilled water
  2. Slowly add in 5ml of glacial acetic acid
  3. Boil for approximately 1 hour
  4. Allow to cool
  5. Filter

Carmalum stain is used for cell nuclei.

Cautions: ALWAYS wear personal protective equipment when in the laboratory with concentrated acids (or any other time)! Always add acid slowly into water and do not add water into acid!

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