Carmalum Stain Recipe

Stock solution

1. Add 10g of carmalum to 200ml of distilled water
2. Slowly add in 5ml of glacial acetic acid
3. Boil for approximately 1 hour
4. Allow to cool
5. Filter

Carmalum stain is used for cell nuclei.

Cautions: ALWAYS wear personal protective equipment when in the laboratory with concentrated acids (or any other time)! Always add acid slowly into water and do not add water into acid!