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HOME > Protocols > Media and Reagents > Clostridium thermocellum Medium Recipe

Clostridium thermocellum Medium Recipe

    1. Dissolve the following in 800ml of distilled water
      • 1mg Resazurin
      • 1.1mg FeSO4 × 7 H2O
      • 6g Na-ß-glycerophosphate
      • 0.13g CaCl2 × 2 H2O
      • 7.2g K2HPO4 × 3 H2O
      • 1.43g KH2PO4
      • 2.6g MgCl2 × 6 H2O
      • 1.3g (NH4)2SO4
      • 0.25g Glutathione
      • 10g Cellulose
      • 4.5g Yeast extract
    2. Bring to 900ml with distilled water
    3. Autoclave to sterilize
    4. Add 50ml of 100g/L filter sterilized Cellobiose (5g)
    5. Aspectically adjust to 1000 with sterile distilled water

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