Desulfovibrio Medium Recipe
- Dissolve the following in 800 ml of distilled water to make Solution A:
q.s. to 980 ml with distilled water
Dissolve the following in 10 ml of distilled water to make Solution B:
Dissolve the following in 10ml of distilled water to make Solution C:
- NH4Cl 1.0 g
- K2HPO4 0.5 g
- Resazurin 1.0 mg
- Yeast extract 1.0 g
- DL-Na-lactate 2.0 g
- MgSO4 x 7 H2O 2.0 g
- CaCl2 x 2 H2O 0.1 g
- Na2SO4 1.0 g
Bring solution A to the boil for a few minutes, then cool to room temperature while gassing with oxygen-free N2 gas.
Add solution B and C, adjust pH to 7.8 with NaOH, and distribute under N2 in anaerobic tubes.
During distribution continuously swirl the medium to keep the grey precipitate suspended.
Autoclave to sterilize.
- Ascorbic acid 0.1 g
- Na-thioglycolate 0.1 g