Desulfovibrio Medium Recipe

1. Dissolve the following in 800 ml of distilled water to make Solution A:
• NH₄Cl 1.0 g
• K₂HPO₄ 0.5 g
• Resazurin 1.0 mg
• Yeast extract 1.0 g
• DL-Na-lactate 2.0 g
• MgSO₄ x 7 H₂O 2.0 g
• CaCl₂ x 2 H₂O 0.1 g
• Na₂SO₄ 1.0 g
2. q.s. to 980 ml with distilled water
3. Dissolve the following in 10 ml of distilled water to make Solution B:
• FeSO₄ x 7 H₂O 0.5 g
4. Dissolve the following in 10ml of distilled water to make Solution C:
• Ascorbic acid 0.1 g
• Na-thioglycolate 0.1 g
5. Bring solution A to the boil for a few minutes, then cool to room temperature while gassing with oxygen-free N₂ gas.
6. Add solution B and C, adjust pH to 7.8 with NaOH, and distribute under N₂ in anaerobic tubes.
7. During distribution continuously swirl the medium to keep the grey precipitate suspended.
8. Autoclave to sterilize.