Coating Buffer Recipe
Dissolve 5.3g of Na2CO3
in 900ml distilled H2O.
Dissolve 4.2g of NaHCO3
in the solution from step 1.
- Dissolve 1g sodium azide in the solution from step
- pH to 9.6.
Adjust volume to 1L with additional
Note: For coating step add 0.1 to 1ug/ml anti target
antibody to buffer and incubate at 4oC for