Flagella Stain Recipe

Flagella Stain is a stain used commonly to allow for the direct observation of flagella.

1. Dissolve 20g of tannic acid in 30ml of 60% ethanol
2. Dissolve 36g of aluminum chloride (hydrated) to the solution from step 1
3. Dissolve 20g of ZnCl₂ in the solution from step 2
4. Dissolve 3g of rosaniline HCl in the solution from step 3
5. q.s. to 70ml with 60% ethanol