Hollande's Fixative Recipe

1. Stock solution
   • 75g Copper acetate (cupric)
   • 3L Distilled water
   • 120g Picric acid
   • 300ml Formaldehyde
   • 45ml Glacial acetic acid

• Note: Small biopsies fix in 2 to 4 hours, large specimens may need up to 3 days.
• Caution: Picric acid can become explosive if allowed to dry out and is toxic through skin
  exposure. Formaldehyde is a carcinogen. Glacial acetic acid is caustic. Use personal protective equipement, prepare under hood in a well ventilated area. Consult with appropriate safety officials at your institution for proper procedures and disposal.