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HOME > Protocols > Agar and Reagents > LB Agar Recipe

LB Agar Recipe

  1. Add the following to 800ml H2O
      • 10g Bacto-tryptone
      • 5g yeast extract
      • 10g NaCl
  2. Adjust pH to 7.5 with NaOH
  3. Add 15g agar
  4. Melt agar into solution in the microwave
  5. Adjust volume to 1L with dH2O
  6. Sterilize by autoclaving

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