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HOME > Protocols > Agar and Reagents > M17 Media Recipe

M17 Media Recipe

  1. Add the following to 800ml H2O
      • 5g Neopeptone
      • 5g Bactosoytone
      • 5g Beef extract
      • 2.5g Yeast extract
      • 0.5g Ascorbic acid
      • 1g 2-Glycerolphosphate x 5 H2O, disodium salt
  2. Adjust volume to 960ml with dH2O
  3. Sterilize by autoclaving
  4. Let cool to room temperature
  5. Add 40ml of 25% glucose
  6. Add 1ml of 1M MgSO4

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