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HOME > Protocols > Media and Reagents > Recipe for 5% Malachite Green

Recipe for 5% Malachite Green

    1. Dissolve the following in 10ml of distilled water
        • 0.5g Malachite green
    2. Stir until crystals are mostly dissolved (~20-30 minutes)
    3. Filter to remove residual crystals

Malachite green is a relatively weakly binding dye for staining bacterial spores. A decolorizer is not necessary for this dye.


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