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HOME > Protocols > Media and Reagents > N4 Mineral Medium Recipe

N4 Mineral Medium Recipe

    1. Combine the following in 900ml water:
      • 1ml Phenol Red (0.04 %)
      • 10ml FeSO4/EDTA solution (see below)
      • 11.92g HEPES buffer (Sigma H-3375)
      • 0.5g NaCl
      • 20mg CaCl2 × 2 H2O
      • 40mg MgSO4 × 7 H2O
      • 0.2g KH2PO4
      • 0.5g (NH4)2SO4
    2. Adjust to 1000ml with water
    3. Autoclave or filter sterilize.

FeSO4/EDTA solution:

  1. Combine the following in 100ml distilled water:
    • 19mg EDTA
    • 18mg FeSO4 × 7 H2O
  2. Autoclave or filter sterilize.

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