SOB Media Recipe (1000 ml)

1. Measure ~900ml of distilled H₂O
2. Add 20g Bacto Tryptone
3. Add 5g Bacto Yeast Extract
4. Add 2ml of 5M NaCl
5. Add 2.5ml of 1M KCl
6. Add 10ml of 1M MgCl₂
7. Add 10ml of 1M MgSO₄
8. Adjust to 1L with distilled H₂O
9. Sterilize by autoclaving