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HOME > Protocols > Media and Reagents > SOB Media Recipe (1000 ml)

SOB Media Recipe (1000 ml)

    1. Measure ~900ml of distilled H2O
    2. Add 20g Bacto Tryptone
    3. Add 5g Bacto Yeast Extract
    4. Add 2ml of 5M NaCl
    5. Add 2.5ml of 1M KCl
    6. Add 10ml of 1M MgCl2
    7. Add 10ml of 1M MgSO4
    8. Adjust to 1L with distilled H2O
    9. Sterilize by autoclaving

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