Starch - Mineral Salt (STMS) Agar Recipe

1. Dissolve the following in 100 ml of distilled water to make trace elements solution:
• ZnSO₄ x 7 H₂O 0.1 g
• MnCl₂ x 4 H₂O 0.1 g
• FeSO₄ x 7 H₂O 0.1 g
2. Add 20g of of rolled oats to 900 ml distilled water
• Agar 15.0 g
• Trace element solution (see above) 1.0 ml
• CaCO₃ 2.0 g
• NaCl 1.0 g
• MgSO₄ x 7 H₂O 1.0 g
• K₂HPO₄ 1.0 g
• (NH₄)₂SO₄ 2.0 g
• Starch, soluble 10.0 g
3. q.s. to 1L with distilled water.
4. Adjust pH to 7.2.
5. Autoclave to sterilize.