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Zenker's Fixative Recipe
- Stock solution
- 50g Mercuric chloride
- 25g Potassium dichromate
- 10g Sodium sulfate
- 1L Distilled water
- Working solution
- 95ml Zenker’s stock
- 5ml Glacial acetic acid
Note: Small biopsies fix in 2 to 4 hours, large specimens may need up to 3 days.
Caution: Mercuric chloride is a severe skin and eye irritant. Potassium dichromate is toxic by inhalation of dust and by ingestion. Glacial acetic acid is caustic. Use personal protective equipement, prepare under hood in a well ventilated area. Consult with appropriate safety officials at your institution for proper procedures and disposal.
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