Cell Staining Buffer Recipe

1. Dissolve 8g of NaCl, 0.2g of KCl, 1.44g of Na₂HPO₄, and 0.24g of KH₂PO₄ in 800ml distilled H₂O.
2. Add 20 ml of heat inactivated FBS.
3. Add 0.9 grams of sodium azide.
4. Adjust pH to 7.4 with HCl.
5. Adjust volume to 1L with additional distilled H₂O.
6. Sterilize by filtration.
7. Store at 4^oC.