Protocol for Freezing of Tissue Culture Cells with Serum

1. Prepare freezing medium as indicated on the freezing media Recipe
2. Harvest cells in log phase growth
3. Count cells be accepted method and centrifuge
4. Suspend the cells in the freezing medium at 5x10⁶ cells/mL
5. Aliquot the cells as appropriate
6. Freeze the cells in a rate controlled freezer or by placing them at -20^oC for approximately 3 hours and at -70^oC overnight
7. Store cells in liquid nitrogen