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HOME > Protocols > Media and Reagents > Cell Culture Freezing Media Recipe

Cell Culture Freezing Media Recipe

  1. Filter sterilize 700ml of RPMI-1640 (2mM L-Glutamine) and 200ml fetal bovine serum (FBS) through a 0.22Ám filter
  2. Add 100ml sterile dimethyl sulfoxide (DMSO)
  3. Aliquot into tubes as appropriate

Caution: Do not filter DMSO, it will dissolve the cellulose acetate membrane. Store the tubes at -80oC for up to one year.

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