Cell Culture Freezing Media Recipe

1. Filter sterilize 700ml of RPMI-1640 (2mM L-Glutamine) and 200ml fetal bovine serum (FBS) through a 0.22µm filter
2. Add 100ml sterile dimethyl sulfoxide (DMSO)
3. Aliquot into tubes as appropriate

Caution: Do not filter DMSO, it will dissolve the cellulose acetate membrane. Store the tubes at -80^oC for up to one year.