Culture Freezing Media Recipe
- Filter sterilize 700ml of RPMI-1640 (2mM L-Glutamine)
and 200ml fetal bovine serum (FBS) through a 0.22Ám
- Add 100ml sterile dimethyl sulfoxide (DMSO)
- Aliquot into tubes as appropriate
Caution: Do not filter DMSO, it will dissolve
the cellulose acetate membrane. Store the tubes at -80oC
for up to one year.