Recipe for Tissue Homogenization Buffer for ELISA

Stock Solution:

1. Weigh out 0.025 grams of Aprotinin and Luepeptin and dissolve in 25ml of water
2. Mix and aliquot into 70ul single use aliquots

To prepare homogenization buffer

1. Add 50ul of stock solution to 100ml of PBS
2. Sterilze by filtration

Final concentration is 0.5ug/ml